NORDIC PULP & PAPER RESEARCH JOURNAL
Home » Volume 32, 2017 » Issue No. 4
SPECIAL ISSUE ON LIGNIN
Variation in susceptibility to microbial lignin oxidation in a set of wheat straw cultivars: influence of genetic, seasonal and environmental factors - OPEN ACCESS
Marina Konstantopoulou, Patrick J. Slator, Charles R. Taylor, Elizabeth M. Wellington, Gordon Allison, Andrea L. Harper, Ian Bancroft and Timothy D.H. Bugg
Abstract
An alkali lignin preparation from wheat straw was found to be compatible with a previously developed nitrated lignin assay for microbial lignin oxidation, allowing comparison of the susceptibility of different wheat lignins to microbial oxidation. Alkali lignin was prepared from a panel of 18 wheat varieties, and was analysed by FT-IR spectroscopy, revealing minor variations in the lignin structure. Samples of nitrated alkali lignin were assayed against five lignin-degrading bacteria: Streptomyces viridosporus, Rhodococcus jostii RHA1, Pseudomonas putida mt-2, Microbacterium phyllosphaerae, and Sphingobacterium sp. T2. Up to 5-fold variation in rates of lignin oxidation were observed between different wheat varieties, and between different bacterial strain, with Sphingobacterium sp. T2 showing consistently highest absorbance changes. Testing of wheat varieties from two consecutive harvests, and from different locations, revealed that the observed variation was due to a combination of genetic, seasonal and environmental factors, but that some wheat varieties showed consistently higher rates of oxidation. Samples of wheat straw treated with Sphingobacterium sp. T2 were analysed by FT-IR spectroscopy after 7 and 14 daysí treatment, showing changes in lignin structure versus time, consistent with lignin breakdown.
Keywords
Pseudomonas putida, Rhodococcus jostii RHA1, Sphingobacterium, Microbial lignin degradation, Wheat straw
Pages
493-507
DOI
10.3183/NPPRJ-2017-32-04-p493-507
Full text
PDF Document N2017-32-04_p493-507_Bugg.pdf (715KB)
Variation in susceptibility to microbial lignin oxidation in a set of wheat straw cultivars: influence of genetic, seasonal and environmental factors - OPEN ACCESS (2017-32-04_p493-507_Bugg.pdf)
Nordic Pulp & Paper Research Journal (NPPRJ) is an international scientific magazine covering science and technology for the areas of wood or bio-mass constituents, pulp and paper and including new fiber-based materials, recovery and by-products from pulping processes, bio-refining and energy issues. Articles meeting required scientific standards are accepted from any continent.
ISSN: 0283-2631